Antibodies to non-functional oligomeric P2X7 receptors and methods of use thereof

An antigen binding site for binding to a P2X7 receptor, the antigen binding site being defined by a general formula comprising:

• FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4-linker-FR1a-CDR1a-FR2a-CDR2a-FR3a-CDR3a-FR4a
• wherein:
• FR1, FR2, FR3, FR4, FR1a, FR2a, FR3a and FR4a are each framework regions;
• CDR1, CDR2, CDR3, CDR1a, CDR2a, CDR3a are each complementarity determining regions;
• wherein:
• CDR1 has an amino acid sequence of KASQNVGTNVA (SEQ ID NO:
• 5)
• CDR2 has an amino acid sequence of SASFRYS (SEQ ID NO:
• 6)
• CDR3 has an amino acid sequence of QQYNSYPFT (SEQ ID NO:
• 33)
• CDR1a has an amino acid sequence of SYYMS (SEQ ID NO:
• 23)
• CDR2a has an amino acid sequence of AINSNGGSTYYPDTVKG (SEQ ID NO:
• 24)
• CDR3a has an amino acid sequence selected from the group consisting of HYSSRFFDV (SEQ ID NO:
• 34), NFKLMYYNV (SEQ ID NO:
• 13), NYRGDYYET (SEQ ID NO:
• 8), NFLESYFEA (SEQ ID NO:
• 7), NYLPMYYEV (SEQ ID NO:
• 12), HYIKVYYEA (SEQ ID NO:
• 15), HYSSRFFEV (SEQ ID NO:
• 16), NFRVMFFKA (SEQ ID NO:
• 17), HFQRGYYNI (SEQ ID NO:
• 10), YHVIQYLGP (SEQ ID NO:
• 19), HYSKEYYNI (SEQ ID NO:
• 9), YFPLVYYDV (SEQ ID NO:
• 11), DFTVPFYNA (SEQ ID NO:
• 20), NYDKKYFDV (SEQ ID NO:
• 21).