Enhanced protein purification through a modified protein A elution

A method for purifying a polypeptide comprising a CH2/CH3 region, comprising the steps of:

• (a) binding the polypeptide to Protein A;
• and
• (b) eluting the polypeptide with a pH gradient starting at or below 5.0 using an elution buffer, wherein the elution buffer contains a high pH buffer and a low pH buffer and wherein the pH gradient is formed by adjusting a percentage of each pH buffer in the elution buffer,
• wherein the high pH buffer is at about 5.0 and wherein the low pH buffer is at about 2.7, wherein the pH gradient ends at about 3.7, and
• wherein an aggregate, a host cell impurity, a virus filter foulant, a virus particle and a virus-like particle are removed from the desired polypeptide.