# US 9,427,466 B2 · Nanoparticle-assisted ultrasound for breast cancer therapy

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- Number: US 9,427,466 B2
- Document ID: US9427466B2
- Status: Granted
- Kind: B2
- Application number: 14/808,940
- Filed: 2015-07-24
- Published: 2015-11-19
- Granted: 2016-08-30
- Earliest claimed: 2013-04-16
- Assignees: Academia Sinica
- Applicants: Academia Sinica
- Inventors: Olga K. Kosheleva; Peter Lai; Nelson G. Chen; Michael Hsiao; Chung-Hsuan Chen
- Web page: https://patentgrep.com/patent/US9427466B2

## CPC

- A61K 41/0033: {Sonodynamic cancer therapy with sonochemically active agents or sonosensitizers, having their cytotoxic effects enhanced through application of ultrasounds (ultrasound therapy per se A61N7/00)}
- A61K 41/0028: {Disruption, e.g. by heat or ultrasounds, sonophysical or sonochemical activation, e.g. thermosensitive or heat-sensitive liposomes, disruption of calculi with a medicinal preparation and ultrasounds}
- A61K 47/6923: {the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb}
- A61N 7/00: Ultrasound therapy (lithotripsy A61B17/22, A61B17/225; massage using supersonic vibration A61H23/00 {; using ultrasound for introducing media into the body A61M37/0092})
- A61N 7/02: Localised ultrasound hyperthermia
- A61P 35/00: Antineoplastic agents
- Y10S 977/773: Nanoparticle, i.e. structure having three dimensions of 100 nm or less
- Y10S 977/911: Cancer cell destruction
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61N: ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY (measurement of bioelectric currents A61B; surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body A61B18/00; anaesthetic apparatus in general A61M; incandescent lamps H01K; infrared radiators for heating H05B)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Methods for reducing the growth of breast cancer cells in a subject by exposing the cells to nanoparticles, and irradiating with a focused, low to medium power ultrasound. The nanoparticles can be gold or magnetic nanoparticles. The nanoparticles can have a cancer drug attached, such as an antibody-based cancer drug.

## Claims

### Claim 1

A method for reducing the growth of breast cancer cells in a subject, the method consisting of the steps:
injecting into the subject magnetic nanoparticles; and
irradiating the subject with focused ultrasound;
wherein the spatial-peak temporal-peak intensity of the ultrasound measured in a well with 1 mL water using a calibrated needle hydrophone is less than 70 W/cm2.

### Claim 2 (depends on 1)

The method of claim 1, wherein the nanoparticles are attached to a cancer drug, a targeted cancer drug, a humanized monoclonal antibody, a chimeric monoclonal antibody, or a fully human antibody.

### Claim 3 (depends on 1)

The method of claim 1, wherein the size of the nanoparticles is from 1 nanometer to 1000 nanometers.

### Claim 4 (depends on 1)

The method of claim 1, wherein the volume of injected nanoparticles is from 0.001 ml/g of subject weight to 0.05 ml/g of subject weight.