Method for rinsing and delivering bone graft

A method comprising:

• obtaining a syringe comprising a filter removably coupled to the syringe, wherein the syringe has bone graft particles and a cryopreservative solution contained therein, and wherein the filter has a pore size such that only a negligible amount of the bone graft particles can pass through;
• depressing a plunger of the syringe to expel the cryopreservative solution through the filter, wherein the bone graft particles are maintained within the syringe;
• washing the bone graft particles, including drawing the plunger to aspirate a wash solution into the syringe, inverting the syringe to mix the wash solution and bone graft particles, and depressing the plunger to expel the wash solution;
• reconstituting the bone graft particles in a reconstitution fluid;
• removing the filter from the syringe;
• and
• depressing the plunger to deliver the bone graft particles through an open bore of the syringe.

A method for rinsing and delivering a bone graft comprising:

• a. selecting a syringe containing bone graft material and a cryopreservative solution, the syringe comprising a proximal plunger, a distal open bore, and a filter removably coupled to the syringe adjacent to the distal open bore, wherein the bone graft material comprises bone particles with diameters of from about 50 μm to about 5 mm and wherein the filter has a pore size of from about 150 μm to about 200 μm;
• b. determining a bone graft material:fluid ratio of from about 10:1 to about 1:1;
• c. depressing the plunger to expel the cryopreservative solution from the syringe and through the filter, wherein the bone graft material is maintained within the syringe;
• d. inserting a distal end of the syringe into a receptacle containing a wash fluid and drawing the plunger to aspirate the wash solution into the syringe, wherein the wash fluid is saline or phosphate buffered saline;
• e. inverting the syringe to mix the wash solution with the bone graft material;
• f. depressing the plunger to expel the wash solution;
• g. repeating d. through f. one or more times to wash the bone graft material;
• h. reconstituting the bone graft material by inserting the distal end of the syringe into a second receptacle containing a reconstitution fluid and drawing the plunger to aspirate the reconstitution fluid to obtain the bone graft material with the predetermined bone graft material:fluid ratio, wherein the reconstitution fluid comprises saline, phosphate buffered saline, whole blood, white blood cells, platelets, platelet rich plasma, platelet poor plasma, bone marrow aspirate, concentrated bone marrow aspirate, or a combination thereof;
• and
• i. removing the filter from the syringe and delivering the reconstituted bone graft material through the open bore to a surgical site by depressing the plunger.