Organic peroxide compounds for microorganism inactivation

A method for preparing a pharmaceutical composition, comprising steps of:

• (i) contacting a microorganism-containing sample with a multifunctional organic peroxide to inactivate microorganisms therein;
• and (ii) preparing a pharmaceutical composition from the product of step (i),
• wherein the multifunctional organic peroxide has a structure of one of the compounds of the following formula:
• where:
• R1a is H;
• R2a is H, C1-6alkyl, C2-6alkenyl, C1-6alkynyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl, amino, N-mono or N-di C1-6alkylated amino, aminocarbonyl, N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl, —(CR4R5)nCR6(OOH)2;
• R3 is H or C(OOH)R1bR2b,
• R1b is H, C1-6alkyl, C2-6alkenyl, C1-6alkynyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl, amino, N-mono or N-di C1-6alkylated amino, aminocarbonyl, N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl,
• R2b is H, C1-6 alkyl, C2-6alkenyl, C1-6alkynyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl amino, N-mono or N-di C1-6alkylated amino, aminocarbonyl, N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl, or
• R2b is linked to R2a by L, wherein L is C1-8alkylene;
• R4 is, at each occurrence, selected from H or C1-3 alkyl, hydroxyl, cyano, nitro, C2-4-alkenyl, C1-3-alkoxy, C2-4-alkenyloxy, C1-3-alkylcarbonyl, carboxy, C1-6-alkoxycarbonyl, aminocarbonyl, N-mono or N-di C1-3alkylated aminocarbonyl, C1-3-thioalkyl, C1-3-alkylsulfinyl, C1-3-alkylsulfonyl, C1-3-alkylaminosulfonyl and di-C1-3-alkylaminosulfonyl;
• R5 is, at each occurrence, selected from H or C1-3 alkyl, hydroxyl, cyano, nitro, C2-4-alkenyl, C1-3-alkoxy, C2-4-alkenyloxy, C1-3-alkylcarbonyl, carboxy, C1-6-alkoxycarbonyl, aminocarbonyl, N-mono or N-di C1-3alkylated aminocarbonyl, C1-3-thioalkyl, C1-3-alkylsulfinyl, C1-3-alkylsulfonyl, C1-3-alkylaminosulfonyl and di-C1-3-alkylaminosulfonyl;
• R6 is H C1-6alkyl, C2-6alkenyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl, amino, N-mono or N-di C1-6alkylated amino, aminocarbonyl, N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl;
• n is 1 to 8;
• and
• wherein the immunogenicity of the microorganisms is not reduced.