Methods for biosynthesizing 1,3 butadiene
AssigneeInvista North America S.A.R.L.
InventorsPaul S. Pearlman, Changlin Chen, Adriana L. Botes, Alex Van Eck Conradie
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Abstract
This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.
Claims25
A method for biosynthesizing butadiene, comprising
- culturing a recombinant microbial host in a culture medium in the presence of a fermentable carbon source;
- enzymatically forming, in the recombinant microbial host, a first terminal vinyl group in 2-butanol using a polypeptide with the activity of a desaturase or a polypeptide with the activity of a monooxygenase to produce 3-buten-2-ol, wherein said desaturase is a cytochrome P450 or said monooxygenase is a gene product of mdpJ;
- and
- enzymatically forming, in the recombinant microbial host, a second terminal vinyl group in 3-buten-2-ol using a polypeptide with the activity of a dehydratase classified under EC 4.2.1- which is capable of accepting 3-buten-2-ol to biosynthesize butadiene by dehydration.
A method of converting a butenol into butadiene, said method comprising culturing a recombinant microbial host in a culture medium comprising a fermentable carbon source, to enzymatically form, in the recombinant microbial host, a first vinyl group in 2-butanol to produce 3-buten-2-ol, and enzymatically form, a second terminal vinyl group in 3-buten-2-ol to produce 1,3-butadiene, said host comprising at least exogenous nucleic acid encoding (i) a polypeptide with the activity of a desaturase or a polypeptide with the activity of a monooxygenase and (ii) a polypeptide with the activity of linalool dehydratase, wherein said polypeptide with the activity of desaturase or said polypeptide with the activity of said monooxygenase enzymatically converts 2-butanol to 3-butene-2-ol, wherein said desaturase is a cytochrome P450 or said monooxygenase is a gene product of mdpJ, and wherein said linalool dehydratase enzymatically coverts 3-buten-2-ol to 1,3-butadiene.
Dependencies are inferred from the claim text.