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US 9,422,532 B2Granted
Nucleic acid encoding N-methylputrescine oxidase and uses thereof
Assignee22nd Century Limited, LLC
InventorsJonathan E. Page, Enwu Liu
Life of this patent
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Abstract
The gene encoding N-methylputrescine oxidase (MPO) and constructs comprising such DNA are provided, including methods of regulating MPO expression independently or with other alkaloid biosynthesis genes to modulate alkaloid production in plants and host cells. MPO genes or fragments thereof are useful for reducing pyrrolidine or tropane alkaloid production in plants, for increasing pyrrolidine or tropane alkaloid production in plants, and for producing an MPO enzyme in host cells.
Claims11
An increased alkaloid plant produced by a method of increasing N-methylputrescine oxidase (MPO) enzyme in a plant comprising:
- (a) introducing into the plant a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- (i) a nucleotide sequence set forth in SEQ ID NO:
- 1;
- (ii) a nucleotide sequence set forth in SEQ ID NO:
- 2;
- (iii) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 3:
- (iv) a nucleotide sequence that is at least 85% identical to the nucleotide sequences of (i), (ii), or (iii) and encodes an MPO enzyme;
- (v) a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of (i), (ii), (iii), or (iv) and encodes an MPO enzyme;
- and
- (vi) a nucleotide sequence that differs from the nucleic acid sequence of (i) or (ii) due to the degeneracy of the genetic code and encodes an MPO enzyme;
- and
- (b) growing the plant under conditions whereby the nucleic acid molecule is expressed thereby increasing levels of MPO enzyme in the plant compared to a control plant grown under similar conditions.
10 more elements
Dependencies are inferred from the claim text.