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AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsLaura K. McMullan, Cynthia Goldsmith, Aubree Kelly, William L. Nicholson, Stuart T. Nichol
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Described herein are the clinical and laboratory characteristics of two patients bitten by ticks and infected with a unique member of the genus Phlebovirus (family Bunyaviridae) with a proposed name of Heartland virus (HRTLV). Provided herein are nucleotide and amino acid sequences of the Phlebovirus isolates, primers and probes that specifically hybridize with the Phlebovirus isolates, and antibodies specific for the Phlebovirus proteins. Also provided are detection assays using the Phlebovirus nucleic acid molecules, proteins, probes, primers and antibodies. Further provided are recombinant Phleboviruses and their use for eliciting an immune response in a subject.
A Phlebovirus polypeptide chemically coupled to a carrier, wherein the amino acid sequence of the polypeptide is at least 95% identical to SEQ ID NO:
An expression vector comprising a nucleic acid molecule, wherein the nucleotide sequence of the nucleic acid molecule is at least 95% identical to SEQ ID NO:
An oligonucleotide 12 to 40 nucleotides in length, wherein the oligonucleotide specifically hybridizes under conditions of high stringency with a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:
An isolated antibody or antigen-binding fragment thereof that specifically binds to a Phlebovirus nucleoprotein (NP), glycoprotein (GP), NSs protein or L protein, wherein the antibody or antigen-binding fragment is conjugated to a detectable label or a magnetic bead, and wherein:
A recombinant Phlebovirus, wherein the genome of the recombinant Phlebovirus comprises:
A Phlebovirus reverse genetics system, comprising a first plasmid comprising an anti-genomic Phlebovirus S segment, a second plasmid comprising an anti-genomic Phlebovirus M segment and a third plasmid comprising an anti-genomic Phlebovirus L segment, wherein each plasmid comprises a T7 promoter and a hepatitis delta virus ribozyme, and wherein:
A Phlebovirus mini-genome reporter system, comprising a first plasmid comprising a reporter gene flanked by UTR sequences of a Phlebovirus S, M or L segment, a second plasmid encoding a Phlebovirus NP, and a third plasmid encoding a Phlebovirus L protein, wherein:
Dependencies are inferred from the claim text.