Back to results
US 9,417,190 B2Granted
Calibrations and controls for droplet-based assays
AssigneeBio-Rad Laboratories, Inc.
InventorsBenjamin J. Hindson, Billy W. Colston, Jr., Kevin D. Ness, Donald A. Masquelier
Life of this patent
Estimating the term…
Abstract
System, including methods and apparatus, for performing droplet-based assays that are controlled and/or calibrated using signals detected from droplets.
Claims5
A method of performing a droplet-based assay, comprising:
- forming droplets from a same mixture, wherein only a subset of the droplets contain a target;
- irradiating a region of a channel;
- detecting a forward scattering signal and a fluorescence signal from a plurality of the droplets as each droplet passes through the region of the channel, wherein the fluorescence signal is detected from a label present in each droplet and varies according to whether the target is present in the droplet;
- identifying accepted droplets of the plurality for which the forward scattering signal meets a condition and rejected droplets of the plurality for which the forward scattering signal does not meet the condition, wherein the condition corresponds to a permitted size of a droplet;
- and
- determining a concentration of the target based on the fluorescence signal from the accepted droplets, and without any contribution of the fluorescence signal from the rejected droplets.
3 more elements
Dependencies are inferred from the claim text.