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US 9,416,378 B2Granted
Biofuel production by recombinant microorganisms
AssigneeThe Regents of the University of California
InventorsJames C. Liao, Shota Atsumi, Kevin M. Smith, Roa Pu Claire Shen, Anthony F. Cann, Michael R. Connor
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Abstract
Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate.
Claims6
A recombinant prokaryote comprising:
- (a) an α-isopropylmalate synthase polypeptide having at least 90% identity to SEQ ID NO:50 and having α-isopropylmalate synthase activity;
- (b) a β-isopropylmalate dehydrogenase polypeptide having at least 90% identity to SEQ ID NO:52 and having β-isopropylmalate dehydrogenase activity;
- (c) an α-isopropylmalate isomerase polypeptide having at least 90% identity to SEQ ID NO:54;
- (d) an α-isopropylmalate isomerase polypeptide having at least 90% identity to SEQ ID NO:56 and having α-isopropylmalate isomerase activity in combination with the α-isopropylmalate isomerase polypeptide of (c);
- (e) a 2-keto-acid decarboxylase polypeptide having at least 90% identity to SEQ ID NO:28 and having 2-keto acid decarboxylase activity;
- (f) a NADH-dependent alcohol dehydrogenase having at least 90% identity to SEQ ID NO:38;
- (g) an acetohydroxy acid synthase polypeptide having at least 90% identity to SEQ ID NO:42;
- (h) an acetohydroxy acid synthase polypeptide having at least 90% identity to SEQ ID NO:40 and having acetohydroxy acid synthase activity in combination with the acetohydroxy acid synthase polypeptide of (g);
- (i) an acetohydroxy acid isomeroreductase polypeptide having at least 90% identity to SEQ ID NO:44 and having acetohydroxy acid isomeroreductase activity;
- and
- (j) a dihydroxy-acid dehydratase polypeptide having at least 90% identity to SEQ ID NO:46 and having dihydroxy-acid dehydratase activity;
- and wherein the recombinant prokaryote produces 3-methyl-1-butanol.
9 more elements
Dependencies are inferred from the claim text.