TaBV transcriptional control element, chimeric constructs and uses therefor

A progeny obtained from a differentiated transgenic plant comprising a chimeric DNA construct, wherein the progeny comprises the chimeric DNA construct, and wherein the differentiated transgenic plant is produced by a method comprising:

• (a) introducing into regenerable plant cells the chimeric DNA construct comprising an isolated DNA molecule comprising a nucleotide sequence with promoter activity that hybridizes under high stringency conditions to the sequence set forth in any one of SEQ ID NO:6, 7 or 8, wherein the nucleotide sequence is operably linked to a foreign or endogenous DNA sequence to be transcribed, so as to yield regenerable transformed plant cells, and wherein the high stringency condition is 1% BSA, 1 mM EDTA, 0.5 M NaHPO4 (pH 7.2), and 7% SDS for hybridization at 65° C. and washing at 68° C. in 0.2×SSC, 0.1% SDS;
• (b) identifying or selecting a population of transformed plant cells;
• and
• (c) regenerating a differentiated transgenic plant from the population.

A plant part obtained from a differentiated transgenic plant produced by a method comprising the steps of:

• (a) introducing into regenerable plant cells a chimeric DNA construct comprising an isolated DNA molecule comprising a nucleotide sequence with promoter activity that hybridizes under high stringency conditions to the sequence set forth in any one of SEQ ID NO:6, 7 or 8, wherein the nucleotide sequence is operably linked to a foreign or endogenous DNA sequence to be transcribed, so as to yield regenerable transformed plant cells, and wherein the high stringency condition is 1% BSA, 1 mM EDTA, 0.5 M NaHPO4 (pH 7.2), and 7% SDS for hybridization at 65° C. and washing at 68° C. in 0.2×SSC, 0.1% SDS;
• (b) identifying or selecting a population of transformed plant cells;
• and
• (c) regenerating a differentiated transgenic plant from the population;
• wherein the plant part contains the chimeric construct.

A differentiated transgenic plant regenerated from transformed plant cells obtained by a method comprising the steps of:

• (a) introducing into regenerable plant cells a chimeric DNA construct comprising an isolated DNA molecule comprising a nucleotide sequence with promoter activity that hybridizes under high stringency conditions to the sequence set forth in any one of SEQ ID NO:6, 7 or 8, wherein the nucleotide sequence is operably linked to a foreign or endogenous DNA sequence to be transcribed, so as to yield regenerable transformed plant cells, and wherein the high stringency condition is 1% BSA, 1 mM EDTA, 0.5 M NaHPO4 (pH 7.2), and 7% SDS for hybridization at 65° C. and washing at 68° C. in 0.2×SSC, 0.1% SDS;
• (b) identifying or selecting a population of transformed plant cells;
• and
• (c) regenerating a differentiated transgenic plant from the population.