Methods and compositions for generating a mouse
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsWilliam Poueymirou, Thomas M. DeChiara, Wojtek Auerbach, David Frendewey, David M. Valenzuela
Life of this patent
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Abstract
Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.
Claims25
An in vitro culture comprising a pre-morula diploid mouse embryo which comprises a mouse donor cell introduced under the zona pellucida, wherein the donor cell comprises and ES cell from an inbred mouse which is heterozygous or homozygous for a genetic modification, wherein a mouse progeny generated from the pre-morula diploid mouse embryo has at least 90% cellular contribution from the ES cell, the in vitro culture further comprising a medium suitable for culturing a mouse ES cell.
A method of generating a mouse embryo comprising:
- (a) introducing a mouse donor cell into a pre-morula host diploid mouse embryo, wherein the donor cell is an ES cell from an inbred mouse;
- (b) culturing the pre-morula host embryo of (a) to the blastocyst stage;
- and
- (c) gestating the embryo of (b) in a surrogate mouse mother, wherein upon gestation a modified mouse embryo is obtained having at least 90% cellular contribution from the mouse ES donor cell.
A method of generating a diploid mouse embryo comprising cells that are homozygous for a genetic modification, comprising:
- (a) introducing a mouse donor cell into a pre-morula host mouse embryo, wherein the mouse donor cell is an ES cell from an inbred mouse and is homozygous for the genetic modification;
- (b) culturing the pre-morula host embryo of (a) to the blastocyst stage;
- and
- (c) gestating the embryo of (b) in a surrogate mouse mother, wherein upon gestation a modified mouse embryo is obtained having at least 90% cellular contribution from the mouse ES donor cell.
A method of generating a diploid mouse embryo comprising cells that are heterozygous for a genetic modification, comprising:
- (a) introducing a mouse donor cell into a pre-morula host mouse embryo, wherein the mouse donor cell is an ES cell from an inbred mouse and is heterozygous for the genetic modification;
- (b) culturing the pre-morula host embryo of (a) to the blastocyst stage;
- and
- (c) gestating the embryo of (b) in a surrogate mouse mother, wherein upon gestation a modified mouse embryo is obtained having at least 90% cellular contribution from the mouse ES donor cell.
Dependencies are inferred from the claim text.