Glucose dehydrogenase
AssigneesARKRAY, Inc., Ultizyme International Ltd.
InventorKoji Sode
Life of this patent
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Abstract
A modified pyrroloquinoline quinone glucose dehydrogenase that exhibits a high selectivity for glucose is provided. A modified pyrroloquinoline quinone glucose dehydrogenase is disclosed in which the amino acid residue G at Position 99 of a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 1, or the amino acid residue G at Position 100 of the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 3, is substituted by the amino acid sequence TGZN (where Z is SX, S, or N and X is any amino acid residue). The modified PQQGDH of the present invention may additionally comprise one or more mutations selected from the group consisting of Q192G, Q192A, or Q192S; L193X; E277X; A318X; Y367A, Y367F, or Y367W; G451C; and N452X (where X is any amino acid residue).
Claims6
A method of analyzing glucose level using a modified pyrroloquinoline quinone glucose dehydrogenase (PGGGDH),
- wherein the amino acid residue G at Position 100 of the PQQGDH represented by SEQ ID NO:
- 3 is substituted by the amino acid sequence TGZN where Z is SX, S, or N, and X is any amino acid residue, and
- optionally from 1 to 10 of amino acid residues at Positions 1 to 99 or at Positions 101 to 480 of SEQ ID NO:
- 3, are substituted with any other amino acid residue(s).
Dependencies are inferred from the claim text.