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AssigneeRaindance Technologies, Inc.
InventorsDarren R. Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
An emulsion library in a single offline container comprising a plurality of pooled aqueous droplets within an immiscible oil comprising at least one surfactant, wherein each droplet comprises the same aqueous fluid and a different primer pair, and wherein each individual primer pair is configured to bind to a different target nucleic acid sequence, and whereby each different target nucleic acid sequence is configured to specifically bind to a different primer pair.
Dependencies are inferred from the claim text.