Compositions and methods of using chondroitinase ABCI mutants
AssigneeAcorda Therapeutics, Inc.
InventorsAnthony O. Caggiano, Andrea Vecchione, Jennifer Iaci
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Abstract
The present disclosure relates to protein and nucleic acid mutants of chondroitinase ABCI. Such nucleic acid mutants encode for chondroitinase ABCI mutant enzymes exhibiting altered chondroitin lyase activity or increased resistance to inactivation from stressors including UV light or heat. Methods of using such nucleic acid mutants encoding chondroitinase ABCI mutant enzymes is also provided.
Claims16
A recombinant nucleic acid sequence encoding a mutant chondroitinase ABC I enzyme, wherein the recombinant nucleic acid sequence comprises SEQ ID NO:
- 9.
An expression vector comprising a recombinant nucleic acid sequence encoding a mutant chondroitinase ABC I enzyme, wherein the recombinant nucleic acid sequence comprises SEQ ID NO:
- 9.
A method of making an expression vector comprising:
- ligating a recombinant nucleic acid sequence to an expression vector, wherein the recombinant nucleic acid sequence comprises SEQ ID NO:
- 9 and encodes a mutant chondroitinase ABC I enzyme.
Dependencies are inferred from the claim text.