Enzymatic systems for carbon fixation and methods of generating same
AssigneeYeda Research and Development Co. Ltd.
InventorsRon Milo, Arren Bar-Even, Elad Noor
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Abstract
A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.
Claims35
A system for carbon fixation, comprising enzymes which catalyze reactions of a carbon fixation pathway, wherein all the carboxylation reactions of the carbon fixation pathway utilize:
- (i) phophoenolpyruvate (PEP) carboxylase and acetyl-CoA carboxylase;
- or
- (ii) phophoenolpyruvate (PEP) carboxylase;
- or
- (iii) pyruvate carboxylase and acetyl-CoA carboxylase;
- or
- (iv) pyruvate carboxylase
- wherein products of said reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, and wherein an additional product of the carbon fixation pathway is glyoxylate, wherein an in-organic carbon is introduced into a substrate to become a carboxylic acid group during said carboylation reactions.
An E. Coli which expresses a system for carbon fixation, comprising enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of said reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of said reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs said carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxylase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an additional product of the carbon fixation pathway is glyoxylate.
Dependencies are inferred from the claim text.