US 9,404,144 B2 · Glucose dehydrogenase

Abstract

An object of the present invention is to provide a novel glucose dehydrogenase, a method for producing the glucose dehydrogenase, and applications of the glucose dehydrogenase. The flavin-binding glucose dehydrogenase of the invention has the following characteristics (1) and (4): (1) Molecular weight: the molecular weight of a polypeptide moiety in the enzyme is about 68 kDa as measured by SDS-polyacrylamide electrophoresis; (2) Km value: the Km value for D-glucose is about 15 mM or less; (3) Temperature stability: stable at a temperature of 55° C. or less; and (4) pH stability: stable at a pH range of 3.0 to 8.5.

Claims14

Dependent claims

A glucose assay kit, comprising:

a flavin-binding glucose dehydrogenase, the flavin-binding glucose dehydrogenase comprising a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO:
1, and having glucose dehydrogenase activity;
and a mediator.

Dependencies are inferred from the claim text.

Facts

Application

14/374,164Filed 7 Feb 2013

Priority

JP 2012-0263929 Feb 2012

Published

US 2015/0031059 A129 Jan 2015

Granted

2 Aug 2016

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12Q 1/54
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12Q 1/32
Involving oxidoreductase: involving dehydrogenase
C12Y 101/05
Oxidoreductases acting on the CH-OH group of donors: with a quinone or similar compound as acceptor

Glucose dehydrogenase

Life of this patent

Glucose dehydrogenase

Life of this patent

Abstract

Claims14