Assays for detecting modified compounds
AssigneeEnzo Life Sciences, Inc.
InventorsElazar Rabbani, Joshua Rabbani, Praveen Pande, Jannis G. Stavrianopoulos
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Abstract
Provided are methods and compositions which are useful for separating, isolating, detecting, and quantifying compounds of interest which have been modified chemically, enzymatically or catalytically from other compounds which have not been so modified. The modifications may take the form of functional groups which are gained, lost or retained by the compounds of interest.
Claims24
A reagent for determining the presence of an enzyme that adds or removes a charged functional group to an uncharged moiety on a substrate or quantifying the activity of said enzyme in a sample, the reagent comprising:
- the substrate to which a first member and a second member of an energy transfer pair are covalently bound at a distance such that a FRET interaction does not occur unless the charged functional group is present,
- wherein the second member of the energy transfer pair has a charge opposite to the charge of the functional group, and
- wherein the presence of the charged functional group on the substrate causes noncovalent binding of the second member of the energy transfer pair to the charged functional group, bringing the first member and the second member of the energy transfer pair close enough so that a FRET interaction occurs.
A reagent for determining the presence of an enzyme that adds or removes an uncharged functional group to a charged moiety on a substrate or quantifying the activity of said enzyme in a sample, the reagent comprising:
- the substrate to which a first member and a second member of an energy transfer pair are covalently bound at a distance such that a FRET interaction does not occur unless the uncharged functional group is absent,
- wherein the second member of the energy transfer pair has a charge opposite to the charge of the moiety, and
- wherein the absence of the uncharged functional group from the substrate causes noncovalent binding of the second member of the energy transfer pair to the charged moiety, bringing the first member and the second member of the energy transfer pair close enough so that a FRET interaction occurs.
Dependencies are inferred from the claim text.