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US 9,404,136 B2Granted
A-xylosidase enhanced conversion of plant biomass into fermentable sugars
AssigneeBoard of Trustees of Michigan State University
InventorsJonathan D. Walton, John S. Scott-Craig, Melissa Borrusch
Life of this patent
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Abstract
The invention relates to increasing the availability of fermentable sugars from plant biomass, such as glucose and xylose. As described herein, α-xylosidases can be employed with cellulases to enhance biomass conversion into free, fermentable sugar residues.
Claims15
A method, comprising:
- a) providing;
- i) a plant biomass comprising a hemicellulose material;
- and
- ii) an enzyme mixture comprising at least about 5% by weight of isolated Aspni5|43342 α-xylosidase, or an α-xylosidase with at least 95% sequence identity to SEQ ID NO:1, and at least 50% by weight of a cellulase mixture comprising cellobiohydrolase, endoglucanase, β-1,4-glucanase, β-glucosidase, endoxylanase, and β-xylosidase;
- and
- b) incubating said biomass with said enzyme mixture using at least about 2 mg of an isolated α-xylosidase per gram biomass for a time and under conditions sufficient to create a depolymerized hemicellulose material;
- wherein said depolymerized hemicellulose material comprises a plurality of free fermentable xylose and glucose residues.
4 more elements
Dependencies are inferred from the claim text.