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US 9,402,890 B2Granted
Methods and compositions for pseudoinfectious alphaviruses
AssigneeThe UAB Research Foundation
InventorsIlya Frolov, Elena Frolova
Life of this patent
Estimating the term…
Abstract
The present invention provides pseudoinfectious alphavirus particles and methods of making them and using them to produce an immune response to an alphavirus in a subject.
Claims11
A pseudoinfectious alphavirus genome encoding:
- a) alphavirus nonstructural proteins nsP1-4,
- b) alphavirus structural proteins E2 and E1, and
- c) a Venezuelan equine encephalitis virus (VEEV) capsid protein comprising the amino acid sequence of SEQ ID NO:2 in which two or more amino acids are substituted, resulting in a phenotype of diminished or eliminated packaging of viral genetic material into an alphavirus particle comprising said alphavirus capsid protein, and wherein the amino acid substitutions change two or more positively charged amino acids selected from R16;
- R23;
- R24:
- R29;
- R53;
- R54;
- K64;
- K65;
- K67;
- K68:
- K73;
- K75;
- K81;
- K82;
- K83:
- K84:
- K88:
- K89:
- K90;
- K92:
- K99:
- K105:
- K106;
- K107;
- K110;
- and/or K111, in any combination, to neutral or negatively charged amino acids.
25 more elements
Dependencies are inferred from the claim text.