Method for detecting nucleosomes containing histone variants
AssigneeSingapore Volition Pte Limited
InventorJacob Vincent Micallef
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Abstract
The invention relates to a method for detecting and measuring the presence of mono-nucleosomes and oligo-nucleosomes and nucleosomes that contain particular histone variants and the use of such measurements for the detection and diagnosis of disease. The invention also relates to a method of identifying histone variant biomarkers for the detection and diagnosis of disease and to biomarkers identified by said method.
Claims10
A method for detecting the presence of a nucleosome containing a histone variant or histone isoform associated with a cell free nucleosome in a body fluid sample which comprises the steps of:
- (i) contacting the body fluid sample with a first binding agent which binds to nucleosomes;
- (ii) contacting the nucleosomes or body fluid sample with a second binding agent which binds to the histone variant or histone isoform;
- (iii) detecting or quantifying the binding of said second binding agent to histone variant or histone isoform in the body fluid sample;
- and
- (iv) using the presence or degree of such binding as a measure of the presence of nucleosomes containing the histone variant or histone isoform in the body fluid sample.
A method for detecting the presence of a nucleosome containing a histone variant or histone isoform associated with a cell free nucleosome in a body fluid sample which comprises the steps of:
- (i) contacting the body fluid sample with a first binding agent which binds to the histone variant or histone isoform;
- (ii) contacting the nucleosomes or body fluid sample with a second binding agent which binds to nucleosomes;
- (iii) detecting or quantifying the binding of said second binding agent to nucleosomes in the body fluid sample;
- and
- (iv) using the presence or degree of such binding as a measure of the presence of nucleosomes containing the histone variant or histone isoform in the body fluid sample.
Dependencies are inferred from the claim text.