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US 9,400,273 B1Granted
7-hydroxycoumarin-based cell-tracking reagents
AssigneeLife Technologies Corporation
InventorsJixiang Liu, Hee Chol Kang, Kyle Gee, Christopher Langsdorf, Jolene Bradford, Gayle Buller
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Abstract
Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.
Claims5
A method for tracking cell proliferation, differentiation, and/or function, the method comprising the steps of:
- a) incubating a mixture of cells and a compound selected from
- or a pharmaceutically acceptable salt thereof;
- b) providing a stimulus to the mixture to elicit a fluorescent signal;
- and
- c) analyzing the stimulated mixture.
2 more elements
Dependencies are inferred from the claim text.