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US 9,399,800 B2Granted
Detecting neoplasm
AssigneeMayo Foundation for Medical Education and Research
InventorsWilliam R. Taylor, Jonathan J. Harrington, Patrick S. Quint, Hongzhi Zou, Harold R. Bergen, III, David I. Smith, David A. Ahlquist
Life of this patent
Estimating the term…
Abstract
This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.
Claims11
A system for detecting colorectal cancer or pre-cancer in a mammal, said system comprising:
- a) a stool sample,
- b) reagents sufficient for measuring a K-ras mutation score within said stool sample, wherein said reagents comprise K-ras specific primers, and
- c) reagents sufficient for converting unmethylated cytosine residues to uracil residues within said stool sample while leaving 5-methylcytosine residues unchanged, wherein said reagents sufficient for converting unmethylated cytosine residues to uracil residues within said stool sample while leaving 5-methylcytosine residues unchanged comprise a bisulfite reagent, and reagents sufficient for measuring BMP3 methylation within said stool sample, wherein said reagents sufficient for measuring BMP3 methylation comprise BMP3 specific primers and a bisulfite reagent.
Dependencies are inferred from the claim text.