Fluid transport in microfluidic applications with sensors for detecting fluid presence and pressure

A system comprising:

• a microfluidic chip comprising:
• a first compartment configured to store a reagent and is fluidly coupled to a second compartment via a first channel;
• a metering chamber disposed along the first channel, wherein the metering chamber comprises a second channel having an inlet section and an outlet section, and wherein the metering chamber is configured to meter a volume of the reagent to the second compartment based on a pressure change of a gas within the second channel;
• and
• a control system communicatively coupled to the microfluidic chip, wherein the control system is configured to provide instructions to one or more valves associated with the microfluidic chip, wherein the one or more valves are configured to control the influx and efflux of the reagent, the gas, or a combination thereof within the metering chamber.

A microfluidic chip comprising:

• a first chamber configured to store a reagent and fluidly coupled to a second chamber via a first channel;
• a metering chamber disposed along the first channel, wherein the metering chamber comprises a second channel having an inlet section and an outlet section, and wherein the outlet section is configured to be coupled to a gas source;
• a first sensor disposed at a measuring site within the inlet section, wherein the first sensor is configured to detect a fluid within the measuring site;
• and
• a second sensor disposed at the outlet section, wherein the second sensor is configured to detect a pressure of the fluid.