Virus-like particles as vaccines for paramyxovirus
AssigneeUniversity of Massachusetts
InventorTrudy Morrison
Life of this patent
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Abstract
The invention provides expression vectors and virus-like particles (VLPs) containing Newcastle Disease Virus Sequences in combination with sequences encoding proteins of interest. The vectors are useful in, for example, generating virus-like particles (VLPs) that contain proteins of interest. In one embodiment, the expressed VLPs elicit an immune response by an animal host against the protein. The invention's VLPs are useful as, for example, vaccines.
Claims23
A non-infectious, replication incompetent, recombinant virus-like particle (VLP) comprising, in operable combination:
- a) Newcastle disease virus (NDV) matrix (M) protein,
- b) NDV nucleocapsid (NP) protein,
- c) a first chimeric protein comprising NDV haemagglutinin-neuraminidase (HN) protein transmembrane domain (TM) protein flanked by Newcastle Disease Virus (NDV) HN protein cytoplasmic domain (CT) protein and by a first protein sequence that comprises a first copy of a heterologous protein of interest, and
- d) a second chimeric protein comprising NDV fusion (F) protein transmembrane domain (TM) protein flanked by NDV F protein cytoplasmic domain (CT) protein and by a second protein sequence that comprises a second copy of said heterologous protein of interest,
- wherein said VLP lacks NDV RNA.
A non-infectious, replication incompetent virus-like particle (VLP) produced by a method comprising:
- a) providing an expression vector comprising, in operable combination:
- 1) a first nucleic acid sequence encoding a Newcastle Disease Virus transmembrane domain (TM) protein,
- 2) a second nucleic acid sequence encoding Newcastle Disease Virus cytoplasmic domain (CT) protein,
- 3) a third nucleic acid sequence encoding a protein of interest, wherein said first nucleic acid sequence is flanked by said second and third nucleic acid sequences, and
- 4) a fourth nucleic acid sequence encoding Newcastle Disease Virus matrix (M) protein,
- b) providing a host cell, and
- c) transfecting said host cell with said vector to produce a virus-like particle (VLP) comprising said protein of interest,
- wherein said VLP lacks NDV RNA.
A non-infectious, replication incompetent, recombinant virus-like particle (VLP) comprising, in operable combination:
- a) Newcastle disease virus (NDV) matrix (M) protein,
- b) NDV nucleocapsid (NP) protein, and
- c) NDV haemagglutinin-neuraminidase (HN) protein transmembrane domain (TM) protein flanked by Newcastle Disease Virus (NDV) HN cytoplasmic domain (CT) protein and by a protein of interest that comprises an ectodomain sequence of RSV G protein,
- wherein said VLP lacks NDV RNA.
Dependencies are inferred from the claim text.