Back to results
US 9,396,921 B2Granted
Method and apparatus for pyrolysis-induced cleavage in peptides and proteins
AssigneeWyoming Research Products Center
InventorsFranco Basile, Shaofeng Zhang
Life of this patent
Estimating the term…
Abstract
A method and apparatus for conducting the rapid pyrolysis of peptides, proteins, polymers, and biological materials. The method can be carried out at atmospheric pressures and takes only about 5 to 30 seconds. The samples are cleaved at the C-terminus of aspartic acid. The apparatus employs a probe on which the sample is heated and digested components analyzed.
Claims5
A method of analyzing a peptide sample containing aspartic acid and cysteine, comprising the steps of:
- (a) heating the peptide sample to between about 180° C. and about 250° C., in a period of between about 5 seconds and about 30 seconds to cleave the peptide at both the aspartic acid and cysteine site-specific locations;
- (b) electrospraying the digested sample with a solvent to produce desorbed ions of components of the digested sample;
- and
- (c) detecting the desorbed ions.
1 more element
Apparatus for analyzing a peptide sample containing aspartic acid and cysteine, comprising:
- (a) a heating element having a surface on which the sample is deposited and which heats the peptide sample to between about 180° C. and about 250° C., in a period of between about 5 seconds and about 30 seconds to cleave the peptide at both the aspartic acid and cysteine site-specific locations;
- (b) an electrospray device that subjects the digested sample to a solvent spray to produce desorbed ions of components of the digested sample;
- and
- (c) a detector for detecting the desorbed ions.
1 more element
Dependencies are inferred from the claim text.