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US 9,394,524 B2Granted
Chemical approaches for generation of induced pluripotent stem cells
AssigneeThe Scripps Research Institute
InventorsTongxiang Lin, Wenlin Li, Sheng Ding
Life of this patent
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Abstract
The present invention provides for identification and use of small molecules to induce pluripotency in mammalian cells as well as other methods of inducing pluripotency.
Claims8
A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising
- (a) introducing into the mammalian non-pluripotent cell, one or more expression cassettes comprising an Oct4 polynucleotide, a Sox2 polynucleotide, and a Klf4 polynucleotide, and
- (b) contacting the cell of step (a) with a TGFβ receptor/ALK5 inhibitor and a GSK-3 inhibitor,
- thereby producing an induced pluripotent stem cell from the mammalian non-pluripotent cell.
Dependencies are inferred from the claim text.