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US 9,389,227 B2Granted
Solid substrate comprising array of dendrons and methods for using the same
AssigneesPosco, Postech Foundation
InventorsJoon Won Park, Bong Jin Hong
Life of this patent
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Abstract
The present invention provides solid supports comprising a surface bound array of dendrons and methods for using the same.
Claims7
An array of dendrons, said array comprising a solid support having at least a first surface;
- and a plurality of conically shaped dendrons covalently attached to said first surface of said solid support, wherein each of said dendron is of the formula:
- Z—R1-Q1-{(R3-Q3)b-[(R4-Q4)c-(R5—Y)x]y}3
- wherein
- c is 0 or 1;
- x is 1 when c is 0 or when c is 1, x is 3;
- y is 1 when b is 0 or when b is 1, y is 3;
- Z is X-O-C(═O)—NH—, wherein X is a nucleic acid probe;
- R1 is —(CH2)3—C(═O)—NH—;
- Q1, Q3 and Q4 are C;
- R3 is —CH2—O—(CH2)2—C(═O)—NH—(CH2)3—C(═O)—NH—;
- R4 is —CH2—O—(CH2)2—C(═O)—NH—;
- R5 is —CH2—O—(CH2)2—;
- Y is a terminal functional group moiety of the formula —COOH;
- wherein a plurality of said terminal functional groups on each of said dendron are covalently attached to said first surface of said solid support, and wherein said dendrons are spaced at regular intervals between about 0.1 nm and about 100 nm, and wherein a discrimination efficiency of the probe is at least 80%.
11 more elements
An array of dendrons, said array comprising a solid support having at least a first surface;
- and a plurality of conically shaped dendrons covalently attached to said first surface of said solid support, wherein each of said dendron is of the formula:
- Z—R1-Q1-[(R4-Q4)c-(R5—Y)x]3 I
- wherein
- c is 0 or 1;
- x is 1 when c is 0 or when c is 1, x is 3;
- Z is X-O-C(═O)—NH—, wherein X is a probe;
- R1 is —(CH2)3—C(═O)—NH—;
- Q1 and Q4 are C;
- R4 is —CH2—O—(CH2)2—C(═O)—NH—;
- R5 is —CH2—O—(CH2)2—;
- Y is a terminal functional group moiety of the formula —COOH;
- wherein a plurality of said terminal functional groups on each of said dendron are covalently attached to said first surface of said solid support, and wherein said dendrons are spaced at regular intervals between about 0.1 nm and about 100 nm, and wherein the discrimination efficiency of the probe is at least 80%.
9 more elements
Dependencies are inferred from the claim text.