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US 9,388,438 B2Granted
Enone reductases
AssigneeCodexis, Inc.
InventorsChristopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
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Abstract
The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
Claims20
A method of reducing an α,β unsaturated compound, the method comprising:
- (a) providing:
- (i) an α,β unsaturated compound selected from the group consisting of an α,β unsaturated ketone, an α,β unsaturated aldehyde, an α,β unsaturated nitrile, and an α,β unsaturated ester;
- and (ii) an enone reductase polypeptide comprising an amino acid sequence that is at least 80% identical to a SEQ ID NO:8, wherein the residue corresponding to X251 is A, C, R, E, D, W, Y, S, V, G, L, or I;
- and
- (b) contacting the α,β unsaturated compound with the enone reductase polypeptide under reaction conditions suitable for conversion of the α,β unsaturated compound to the corresponding saturated product compound selected from the group consisting of a saturated ketone, a saturated aldehyde, a saturated nitrile, and a saturated ester.
2 more elements
Dependencies are inferred from the claim text.