Enhanced production of isoprene using host cells having decreased ispA activity
AssigneesDanisco US Inc., The Goodyear Tire & Rubber Company
InventorsJoseph C. McAuliffe, Rachel E. Muir, Alex T. Nielsen, Caroline M. Peres, Dmitrii V. Vaviline, Derek H. Wells
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Abstract
This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.
Claims43
Recombinant microbial cells capable of producing isoprene, wherein said cells comprise
- (i) a geranyltranstransferase or farnesyl diphosphate synthase (ispA) gene having decreased functional activity and
- (ii) one or more nucleic acids encoding:
- (a) an isoprene synthase polypeptide, wherein the isoprene synthase polypeptide is encoded by a heterologous nucleic acid;
- and (b) one or more mevalonate (MVA) pathway polypeptides, and (c) a polypeptide having phosphoketolase activity, wherein culturing of said recombinant cells in a suitable media provides for the production of said polypeptides and synthesis of isoprene.
Dependencies are inferred from the claim text.