Methods and apparatus for gas stream mass transfer with a liquid
AssigneeLife Technologies Corporation
InventorsChristopher D. Brau, Nephi D. Jones, Benjamin R. Madsen, Michael E. Goodwin
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Abstract
A method and system for achieving a gas-liquid mass transfer includes delivering into a compartment of a container a liquid, the liquid having an exposed top surface disposed within the compartment. A stream of a gas is passed over the top surface of the liquid so that the stream of gas produces turbulence on the top surface that is sufficient to achieve the gas-liquid mass transfer. In one embodiment the liquid is a culture that includes cells or microorganisms and the mass transfer functions to oxygenate the culture sufficient to sustain the cells or microorganisms.
Claims17
A method for oxygenating a culture of cells or microorganisms, the method comprising:
- a) delivering into a compartment of a container a culture comprised of (i) cells or microorganisms and (ii) medium, the culture having an exposed top surface disposed within the compartment;
- b) mixing the culture within the container;
- and
- c) blowing a stream of a gas containing oxygen over at least a portion of the top surface of the culture while the culture is being mixed so that the blowing stream of gas produces turbulence on the top surface that is sufficient to produce a mass transfer of oxygen between the gas and the culture, the mass transfer being sufficient to oxygenate the culture for growing the cells or microorganisms.
Dependencies are inferred from the claim text.