Microscale bioprocessing system and method for protein manufacturing

A bioprocessing system, comprising:

• a reactor adapted for cell-free protein expression that produces a protein utilizing cell-free protein expression;
• at least one sensor for monitoring reaction parameters in the reactor;
• and
• a purification module for receiving the protein from the reactor and for purifying the protein from reagents, wherein the purification module comprises,
• a chromatography component for receiving the protein from the production module and for outputting purified protein, and
• a diafiltration component comprising a product section for receiving the purified protein from the chromatography component and a buffer section for receiving a buffer solution, wherein the diafiltration component outputs further purified protein, and wherein the diafiltration component further comprises,
• a first substrate,
• a second substrate, and
• a diafiltration membrane positioned between the first and second substrates.