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US 9,382,517 B2Granted
HCV full-length infectious cell culture systems and applications thereof
AssigneesHvidovre Hospital, Københavns Universitet
InventorsYiping Li, Troels Kasper Høyer Scheel, Santseharay Ramirez Almeida, Judith M. Gottwein, Jens Bukh
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Abstract
The present invention relates to nucleic acid sequences that encode hepatitis C viruses (HCV) that are useful in the fundamental research of HCV as well as in the search of a vaccine against HCV. In particular the present invention relates to nucleic acid sequences that comprises HCVs which are capable of expressing said virus when transfected into cells and are capable of infectivity in vivo.
Claims20
An isolated nucleic acid molecule, which encodes a human hepatitis C virus, wherein said molecule:
- (i) is capable of expressing said virus when transfected into cells,
- (ii) is capable of infectivity in vivo,
- (iii) comprises at least one adaptive mutation in the amino acid sequence of NS3, which is F1464L,
- (iv) comprises at least one adaptive mutation in the amino acid sequence of NS4A, which is A1672S, and
- (v) comprises at least one adaptive mutation in the amino acid sequence of NS5B, which is D2979G,
- wherein the amino acid positions of the adaptive mutations are given with reference to the sequence provided at GenBank accession number AF009606.
3 more elements
Dependencies are inferred from the claim text.