Adsorptive membranes for trapping viruses
AssigneeWisconsin Alumni Research Foundation
InventorMark R. Etzel
Life of this patent
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Abstract
A disposable, virus-trapping membrane, and a corresponding method to remove viruses from solution are described. The membrane includes a disposable, micro-porous filter membrane and a ligand immobilized on the membrane. The ligand irreversibly and selectively binds viruses. The ligand also has a pKa sufficiently high to repel antibodies via electrostatic charge repulsion.
Claims22
A disposable, virus-trapping membrane comprising:
- a disposable, micro-porous filter membrane;
- and
- a multi-modal anion-exchange ligand immobilized on the membrane, wherein the ligand is dimensioned and configured to:
- (a) bind neutral viruses;
- (b) have a pKa sufficiently high to repel basic proteins via electrostatic charge repulsion;
- and
- (c) yield a log-reduction value (LRV) of at least 1.0 for the neutral viruses disposed in a solution comprising 50 mM salt.
A disposable, virus-trapping membrane comprising:
- a disposable, micro-porous filter membrane;
- and
- a ligand immobilized on the membrane, wherein the ligand is selected from the group consisting of tyrosinol, tryptophanol, octopamine, 2-aminobenzimidazole, 1,3-diamino-2-hydroxypropane, tris(2-aminoethyl)amine, and agmatine, and further wherein the ligand:
- (a) binds neutral viruses;
- (b) is a multi-modal anion-exchange ligand;
- (c) has a pKa sufficiently high to repel basic proteins via electrostatic charge repulsion;
- and
- (d) yields a log-reduction value (LRV) of at least 1.0 for the neutral viruses disposed in a solution comprising 50 mM salt.
A method of removing viruses from a solution suspected of containing viruses, the method comprising:
- contacting a solution suspected of containing viruses with virus-trapping membrane comprising a disposable, micro-porous filter membrane and a multi-modal anion-exchange ligand immobilized on the membrane, wherein the ligand is dimensioned and configured to bind neutral viruses, has a pKa sufficiently high to repel basic proteins present in the solution via electrostatic charge repulsion, and yields a log-reduction value (LRV) of at least 1.0 for the neutral viruses disposed in a solution comprising 50 mM salt.
A disposable, virus-trapping membrane comprising:
- a disposable, micro-porous filter membrane;
- a multi-modal exchange ligand immobilized on the membrane, wherein the ligand is selected from the group consisting of tryptophanol, 2-aminobenzimidazole, tris(2-aminoethyl)amine, and agmatine, and further wherein the ligand is dimensioned and configured to:
- (a) bind neutral viruses;
- (b) have a pKa sufficiently high to repel basic proteins via electrostatic charge repulsion;
- and
- (c) yield a log-reduction value (LRV) of at least 1.0 for the neutral viruses disposed in a solution comprising 150 mM salt.
Dependencies are inferred from the claim text.