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US 9,365,875 B2Granted
3-hydroxypropionic acid production by recombinant yeasts
AssigneeNovozymes, Inc.
InventorMariah Bindel
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Abstract
Provided herein are recombinant yeast cells having an active 3-Hydroxypropionic Acid (3-HP) pathway and further comprising a heterologous polynucleotide encoding a non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN). Also described are methods of using the recombinant yeast cells to produce 3-HP and acrylic acid.
Claims27
A recombinant yeast cell, comprising:
- (1) an active 3-hydroxypropionic acid (3-HP) pathway that proceeds through a β-alanine intermediate, and (2) a heterologous polynucleotide encoding a non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN), wherein the cell is capable of producing 3-HP.
A recombinant yeast cell, comprising (1) an active 3-HP pathway, and (2) a heterologous polynucleotide encoding a non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN), wherein the GAPN has:
- (a) at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 192;
- or
- (b) at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 194;
- and
- wherein the cell is capable of producing 3-HP.
4 more elements
Dependencies are inferred from the claim text.