Vector utilizing borna disease virus and use thereof
AssigneeKyoto University
InventorsKeizo Tomonaga, Takuji Daito, Tomoyuki Honda
Life of this patent
Estimating the term…
Abstract
Disclosed is a viral vector comprising (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, and having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c).
Claims5
A viral vector comprising:
- (a) a cDNA of a recombinant viral RNA
- having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome,
- having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene,
- containing restriction enzyme sites both at the 3′ end and at the 5′ end of the foreign gene,
- containing the sequence shown in SEQ ID NO:
- 11 (1) both in the region between the restriction enzyme site at the 3′ end of the foreign gene and the open reading frame of the P gene and in the region between the restriction enzyme site at the 5′ end of the foreign gene and the open reading frame of the L gene, or (2) both in the region between the restriction enzyme site at the 3′ end of the foreign gene and the open reading frame of the P gene and the region between the restriction enzyme site at the 5′ end of the foreign gene and the open reading frame of the M gene when the M gene of the Borna disease viral genome is contained in the same order as that in the Borna disease viral genome, and
- containing at least a base sequence cc inserted in the region between the restriction enzyme site at the 3′ end of the foreign gene and the sequence shown in SEQ ID NO:
- 11, and at least a base sequence cca in the region between the restriction enzyme site at the 5′ end of the foreign gene and the sequence shown in SEQ ID NO:
- 11,
- (b) a DNA encoding a ribozyme, and
- (c) a promoter sequence,
- each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c),
- wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene and the M gene of the Borna disease virus genome are destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, and
- wherein an intron of the L gene of the Borna disease virus genome is deleted from (a) the cDNA of a recombinant viral RNA.
Dependencies are inferred from the claim text.