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US 9,365,825 B2Granted
Expansion of adult stem cells in vitro
AssigneeTaiga Biotechnologies, Inc.
InventorsBrian Curtis Turner, Yosef Refaeli, Gregory Bird
Life of this patent
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Abstract
Disclosed are methods for manipulating and expanding stem cell populations, including adult stem cells, the cells produced by such methods, and various protein constructs related thereto.
Claims29
A method for producing a population of conditionally immortalized adult stem cells comprising:
- providing one or more adult stem cells with
- a) an exogenously synthesized Myc-fusion polypeptide that promotes one or more of cell survival or proliferation;
- and
- b) an exogenously synthesized Bcl-2 domain polypeptide that inhibits apoptosis;
- and
- wherein the Myc-fusion polypeptide is provided to the one or more adult stem cells at intervals of at least 24 hours, and wherein the Myc-fusion polypeptide is continuously provided to the cells for at least 24 hours;
- wherein the Bcl-2 domain polypeptide is provided to the one or more adult stem cells at intervals of at least 24 hours, so as to produce a population of conditionally immortalized adult stem cells;
- and wherein the Myc-fusion polypeptide comprises a Myc polypeptide fused to a protein transduction domain.
5 more elements
A method for producing a population of conditionally immortalized adult stem cells comprising:
- providing one or more adult stem cells with
- a) an exogenously synthesized Myc-fusion polypeptide that promotes one or more of cell survival or proliferation, wherein the Myc-fusion polypeptide is provided at a concentration of about 1 ug/ml to about 50 ug/ml;
- and
- b) an exogenously synthesized Bcl-2 domain polypeptide that inhibits apoptosis, wherein the Bcl-2 domain polypeptide is provided at a concentration of about 1 ug/ml to about 50 ug/ml;
- and
- wherein the Myc-fusion polypeptide is provided to the one or more adult stem cells at intervals of at least 24 hours;
- wherein the Bcl-2 domain polypeptide is provided to the one or more adult stem cells at intervals of at least 24 hours, so as to produce a population of conditionally immortalized adult stem cells;
- and wherein the Myc-fusion polypeptide comprises a Myc polypeptide fused to a protein transduction domain.
5 more elements
Dependencies are inferred from the claim text.