Somatostatin 2 receptor (sst2), deoxycytidine kinase (dck) and uridine monophosphate kinase (umk) pancreatic gene therapy

A pharmaceutical composition comprising an expression vector comprising:

• (i) at least one nucleic acid sequence encoding for a somatostatin 2 receptor protein (sst2) comprising the amino acid sequence SEQ ID NO:
• 1, SEQ ID NO:
• 4, or SEQ ID NO:
• 5;
• (ii) at least one nucleic acid sequence encoding for a deoxycytidine kinase protein (dck) comprising the amino acid sequence SEQ ID NO:
• 2, SEQ ID NO:
• 6, SEQ ID NO:
• 7, or SEQ ID NO:
• 8;
• (iii) at least one nucleic acid sequence encoding for a uridine monophosphate kinase protein (umk) comprising the amino acid sequence SEQ ID NO:
• 3 or SEQ ID NO:
• 9.

A plasmid vector comprising one expression vector comprising a nucleic acid having the sequence set forth in SEQ ID NO:11, which comprises:

• (i) at least one nucleic acid sequence encoding for a somatostatin 2 receptor protein (sst2) comprising the amino acid sequence SEQ ID NO:
• 1, SEQ ID NO:
• 4, or SEQ ID NO:
• 5;
• (ii) at least one nucleic acid sequence encoding for a deoxycytidine kinase protein (dck) comprising the amino acid sequence SEQ ID NO:
• 2, SEQ ID NO:
• 6, SEQ ID NO:
• 7, or SEQ ID NO:
• 8;
• (iii) at least one nucleic acid sequence encoding for a uridine monophosphate kinase protein (umk) comprising the amino acid sequence SEQ ID NO:
• 3 or SEQ ID NO:
• 9, wherein the at least one nucleic acid of (ii)-(iii) are linked by a nucleic acid encoding a cleavable Foot-and-Mouth-Disease virus (FMDV) 2 A peptide, thereby encoding the amino acid sequence set forth in SEQ ID NO:
• 10.