Methods and compositions for diagnosis and prognosis of renal injury and renal failure

A system for evaluating biomarker levels, comprising:

• one or more reagents selected from the group consisting of a reagent which specifically binds TIMP2 and a reagent which specifically binds IGFBP7;
• and
• an assay instrument configured to receive a urine sample and contact the one or more reagents with the urine sample and to generate one or more measured values resulting from binding of TIMP2 and/or IGFBP7 to a respective specific binding reagent in the one or more reagents, the measured values indicative of a TIMP2 concentration and/or a IGFBP7 concentration,
• wherein the assay instrument is further configured to receive entry of, or determine, one or more measured values for one or more of a serum creatinine concentration and a urine output, and
• wherein the assay instrument combines the measured values obtained by the instrument into a single value to provide an assay result.