Method for biotechnological production of dihydrochalcones

Method for production of a dihydrochalcone using a transgenic microorganism comprising:

• (i) providing a transgenic microorganism containing transgenes, said transgenes comprising
• a nucleic acid section (a), comprising a gene coding for a bacterial chalcone isomerase, or a nucleic acid section (a′), comprising a gene coding for a plant chalcone isomerase, or both nucleic acid section (a) and nucleic acid section (a′);
• and
• a nucleic acid section (b), comprising a gene coding for an enoate reductase from E. ramulus operative to form a dihydrochalcone as a product;
• (ii) adding one or more flavanones and optionally one or more precursors or one or more derivatives thereof to the transgenic microorganism and cultivation of the transgenic microorganism under conditions which allow the conversion of the flavanone(s) and/or the precursor(s) or of the derivative(s) thereof to a dihydrochalcone;
• and
• (iii) optionally isolating and purifying the dihydrochalcone.

Method for production of a dihydrochalcone using a transgenic microorganism, comprising the following steps:

• (i) providing a transgenic microorganism containing transgenes, said transgenes comprising
• a nucleic acid section (a), comprising a gene coding for a bacterial chalcone isomerase, or a nucleic acid section (a′), comprising a gene coding for a plant chalcone isomerase, or both nucleic acid section (a) and nucleic acid section (a′);
• and
• a nucleic acid section (b), comprising a gene coding for an enoate reductase from E. ramulus operative to form a dihydrochalcone as a product
• (ii) adding one or more flavanones and optionally one or more precursors or one or more derivatives thereof, to the transgenic microorganism and cultivation of the transgenic microorganism under conditions which allow the conversion of the flavanone(s) and/or the precursor(s) or of the derivative(s) thereof to a dihydrochalcone;
• and
• (iii) isolating and purifying the dihydrochalcone.