Glucose dehydrogenase/cytochrome fusion protein
AssigneesARKRAY, Inc., Ultizyme International Ltd.
InventorKoji Sode
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Abstract
A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome is disclosed. PQQGDH is, for example, a water-soluble PQQGDH derived from Acinetobacter calcoaceticus. The cytochrome is, for example, an electron transfer domain of quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni. The fusion protein of the present invention shows intramolecular electron transfer from PQQ, a redox center, to the cytochrome, which allow construction of a direct electron transfer-type glucose sensor which requires no electron mediators.
Claims15
A fusion protein of pyrroloquinoline quione glucose dehydrogenase (PQQGDH) and a cytochrome, the fusion protein comprising a the linker comprising the amino acid sequence from position 481 to position 504 in SEQ ID NO:
- 2, wherein the linker links PQQGDH to the cytochrome within the fusion protein.
Dependencies are inferred from the claim text.