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US 9,353,386 B2Granted
Means and methods for producing propanediol
AssigneeB.R.A.I.N. Biotechnology Research and Information Network AG
InventorsDaniel Koch, Guido Meurer, Jürgen Eck
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Abstract
The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and/or 1,2-propanediol, said method comprising culturing said host cell and/or utilizing said enzyme to produce said compound.
Claims5
A host cell engineered with one or more nucleic acids encoding
- (a) an enzyme having lactoyl CoA-reductase activity, wherein said enzyme with lactoyl-CoA reductase activity is selected from succinyl-CoA reductases, malonyl-CoA reductases, butyryl-CoA reductases, propionyl-CoA reductases and acetyl-CoA reductases;
- and
- (b) lactaldehyde reductase;
- wherein said engineered host cell has an elevated expression or activity of the enzymes encoded by said nucleic acids, as compared with the parent cell from which it has been derived.
1 more element
A kit comprising
- (a) an enzyme with lactoyl-CoA reductase activity, wherein said enzyme with lactoyl-CoA reductase activity is selected from succinyl-CoA reductases, malonyl-CoA reductases, butyryl-CoA reductases, propionyl-CoA reductases and acetyl-CoA reductases;
- and
- (b) lactaldehyde reductase;
- or one or more nucleic acids encoding said enzymes according to (a) and (b).
1 more element
Dependencies are inferred from the claim text.