Modified gram positive bacteria and uses thereof
AssigneeIntrexon Actobiotics NV
InventorsLothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Abstract
The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.
Claims14
A medicament comprising a bacterium selected from the group consisting of lactic acid bacteria and Bifidobacterium species lacking cellobiose-specific PTS system IIC component (PtcC) activity, wherein the bacterium overexpresses a gene encoding a trehalose transporter, and wherein a gene encoding endogenous TrePP has been partially or completely deleted, disrupted or inactivated.
Dependencies are inferred from the claim text.