Back to results
US 9,346,902 B2Granted
Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 3-hydroxyisobutyrate or methacrylic acid related thereto
AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
Life of this patent
Estimating the term…
Abstract
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.
Claims18
A non-naturally occurring E. coli comprising
- (A) a methanol metabolic pathway having a methanol dehydrogenase and at least one exogenous nucleic acid encoding a methanol dehydrogenase expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of methanol, and
- (B) a 3-hydroxyisobutyrate pathway comprising
- (1) (i) a succinyl-CoA transferase, ligase, or synthetase;
- (ii) a methylmalonyl-CoA mutase;
- (iii) a methylmalonyl-CoA epimerase;
- (iv) a methylmalonyl-CoA reductase (aldehyde forming);
- and (v) a methylmalonate semialdehyde reductase;
- (2) (i) a succinyl-CoA transferase, ligase, or synthetase;
- (ii) a methylmalonyl-CoA mutase;
- (iii) a methylmalonyl-CoA reductase (aldehyde forming);
- and (iv) a methylmalonate semialdehyde reductase;
- or
- (3) (i) a succinyl-CoA transferase, ligase, or synthetase;
- (ii) a methylmalonyl-CoA mutase;
- and (iii) a methylmalonyl-CoA reductase (alcohol forming).
12 more elements
Dependencies are inferred from the claim text.