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US 9,341,594 B2Granted
Manipulation of microfluidic droplets
AssigneeRaindance Technologies, Inc.
InventorsBenjamin J. Miller, Brian Hutchison, Andrew Wilson, Jonathan William Larson, Qun Zhong, Yevgeny Yurkovetsky, Darren R. Link, Mark Weary
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Abstract
The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.
Claims20
A method for measuring droplet frequency comprising the steps of:
- a) flowing a plurality of droplets in a stream of immiscible fluid;
- b) exposing the droplets to a plurality of pulses of illumination, wherein the pulses of illumination are separated by a first duration of time;
- c) capturing an image of the droplets over the plurality of pulses of illumination, wherein the image comprises an exposure from each of the pulses of illumination;
- and
- d) calculating a droplet frequency for the plurality of droplets from the captured image.
2 more elements
Dependencies are inferred from the claim text.