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AssigneeSample6 Technologies, Inc.
InventorsMichael Sandor Koeris, Robert Patrick Shivers, Daniel Robert Brownell, Jason Wyatt Holder, Jayson Linn Bowers
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Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
A composition comprising A511, LP40, and LP124 recombinant Listeria phage, each recombinant phage comprising a heterologous nucleic acid sequence encoding a luciferase that is at least 70% identical to SEQ ID NO:
An article of manufacture comprising A511, LP40, and LP124 recombinant Listeria phage, each recombinant phage comprising a heterologous nucleic acid sequence encoding a luciferase that is at least 70% identical to SEQ ID NOs 4, a β-galactosidase, or a horseradish peroxidase;
Dependencies are inferred from the claim text.