Recombinant yeast and substance production method using the same
AssigneeToyota Jidosha Kabushiki Kaisha
InventorsMasayoshi Muramatsu, Masakazu Ito
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Abstract
Substance productivity is improved by introducing a metabolic pathway for synthesis of acetyl-CoA or acetic acid from glucose-6-phosphate into yeast. Acetic acid productivity, acetyl-CoA productivity, and productivity of a substance made from acetyl-CoA-derived are improved by attenuating genes involved in the glycolytic system of yeast and introducing a phosphoketolase gene into the yeast.
Claims3
A recombinant, non-xylose fermenting Saccharomyces cerevisiae, which comprises an attenuated phosphofructokinase gene endogenous to said Saccharomyces cerevisiae and an introduced phosphoketolase gene, wherein the expression of a Saccharomyces cerevisiae glucose-6-phosphate dehydrogenase gene and/or a Saccharomyces cerevisiae D-ribulose-5-phosphate-3-epimerase gene is enhanced, wherein acetic acid production by said recombinant Saccharomyces cerevisiae is enhanced compared with the wild-type strain, and wherein said introduced phosphoketolase gene encodes the amino acid sequence of any one of SEQ ID NOs:
- 1-19.
Dependencies are inferred from the claim text.