Fluorochromes for organelle tracing and multi-color imaging
AssigneeEnzo Life Sciences, Inc.
InventorsWayne Forrest Patton, Praveen Pande, Yuejun Xiang, Zaiguo Li, James J. Donegan, Elazar Rabbani
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Abstract
Provided are compounds, methods and kits for identifying in cells of interest organelles including nuclei and a wide variety of organelles other than nuclei (non-nuclear organelles), as well as cell regions or cell domains. These compounds and methods can be used with other conventional detection reagents for identifying the location or position or quantity of organelles and even for distinguishing between organelles in cells of interest.
Claims6
A compound selected from:
The compound having the structure
A compound comprising two organelle-targeting fluorochrome compounds having the structure:
- wherein R1-R4 and R5-R8 are independently selected from
- H,
- OH,
- a halide,
- a nitro group,
- a substituted amine,
- a CN group,
- a charged group comprising a salt of an organic acid, an onium group or a protonated amine, wherein
- said salt of an organic acid comprises a sulfate, a sulfonate, a phosphate, a phosphonate, a carboxylate, a borate, or a combination thereof, and
- said onium group comprises a quaternary ammonium, a sulfonium, a phosphonium, or a combination thereof,
- a substituted or unsubstituted alkyl or alkenyl group,
- a substituted or unsubstituted amine, and
- a thiourea group;
- wherein only one member of R1-R8 comprises the structure NH-A-RaRb, wherein A is a C2-8 alkylene group and Ra and Rb are independently selected from H, a C1-4 alkyl, a C2-4 hydroxyalkyl and a C2-4 aminoalkyl;
- and
- wherein said two or more compounds are joined together through a linkage group comprising a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted heteroalkyl group, a substituted or unsubstituted heteroalkenyl group comprising an amide, or one or more substituted or unsubstituted aromatic rings.
A compound selected from the group consisting of
Dependencies are inferred from the claim text.