Removal of arsenic using a dissimilatory arsenic reductase
AssigneeUniwersytet Warszawski
InventorsLukasz Drewniak, Aleksandra Sklodowska, Monika Radlinska, Robert Stasiuk
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Abstract
The object of the invention is the plasmid pSheB, particularly a plasmid which may comprise a fragment of pSheB including the arr module and functional derivatives thereof, and strains containing such a plasmid, preferably the Shewanella sp. strain, deposited as KKP 2045p, which are capable of removing arsenic by dissolution of minerals and reduction of arsenates to arsenites. The object of the invention is also the method and the use of such bacterial strains or compositions which may comprise them, for the selective removal of arsenic from mineral resources, raw materials industry waste or soil.
Claims21
An isolated plasmid comprising a nucleotides 63978 to 72599 of SEQ ID NO:
- 1.
An isolated plasmid pSheB, having the sequence shown in SEQ ID NO:
- 1.
A bacterial strain comprising a nucleotides 63978 to 72599 of SEQ ID NO:
- 1.
A method for selective removal of arsenic from mineral resources, raw materials industry waste or soil, wherein the step of dissimilatory arsenate reduction is carried out with a bacterial strain comprising nucleotides 63978 to 72599 of SEQ ID NO:
- 1, wherein the method comprises:
- a) preparing the mineral resources, waste or soil and mixing with an appropriate culture medium enabling the cultivation of the strain,
- b) adding an inoculum of this strain and culturing under conditions enabling growth and conduction of dissimilatory arsenate reduction,
- whereby arsenic is selectively removed from the mineral resources, waste or soil.
A method for selective arsenic removal from a variety of mineral resources, raw materials industry wastes or soils, wherein the removal of arsenic is carried out by dissimilatory arsenate reduction with a bacterial strain comprising the plasmid pSheB, having the sequence shown in SEQ ID NO:
- 1 wherein the method comprises:
- a) preparing the mineral resources, wastes or soils and mixing with an appropriate culture medium enabling the cultivation of the strain,
- b) adding an inoculum of this strain and culturing under conditions enabling growth and conduction of dissimilatory arsenate reduction,
- whereby arsenic is selectively removed from the mineral resources, wastes or soils.
Dependencies are inferred from the claim text.