Coryneform bacterium transformant and process for producing phenol using the same
AssigneeGreen Phenol Development Co., Ltd.
InventorsHideaki Yukawa, Masayuki Inui
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Abstract
A phenol-producing transformant is provided which can efficiently produce phenol from a saccharide. A gene which encodes an enzyme having tyrosine phenol-lyase activity is transferred into Corynebacterium glutamicum as a host. A process for producing phenol is provided having a step of reacting the transformant in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.
Claims8
A phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a Corynebacterium as a host,
- wherein the gene which encodes an enzyme having tyrosine phenol-lyase activity is a DNA consisting of the nucleotide sequence of SEQ ID NO:
- 36, SEQ ID NO:
- 39, SEQ ID NO:
- 42, SEQ ID NO:
- 45, SEQ ID NO:
- 48 or SEQ ID NO:
- 51;
- a gene which encodes an enzyme having DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate) synthase activity aroG of the Cornybacterium and a gene which encodes an enzyme having chorismate mutase activity of the Cornyebacterium as the host are highly expressed, and
- a gene which encodes an enzyme having prephenate dehydratase activity on the chromosome of the Cornyebacterium as the host is disrupted or deleted.
A Corynebacterium glutamicum transformant PHE7 (Accession Number:
- NITE BP-976).
Dependencies are inferred from the claim text.